Molecular characterization of ESBL- producing uropathogenic Escherichia coli recovered from urine samples of patients attending a University Teaching hospital in Nigeria

Abstract Infection of the urinary tract ranks as one most common infections affecting people worldwide and its treatment is made complicated by rising incidence antibiotic resistance. This study aimed to detect extended spectrum beta-lactamase (ESBL) genes resistance profile uropathogenic Escherichia coli ( E. ) recovered from patients attending a University Teaching hospital in Nigeria. Uropathogenic isolates were obtained culture collection Department Microbiology Parasitology for period four months (October 2019–January, 2020). Antibiotic susceptibility testing was done using disc diffusion method while phenotypic ESBL production detected double synergy test (DDST). Detection β-lactamase Real-Time PCR. Forty-nine 120 urine samples, with 24 (49%) being positive. The antibiotics producers was: ciprofloxacin (100%), cefotaxime cefpodoxime tetracycline (95.7%), ceftazidime (56.7%), amoxicillin-clavulanate (50%), gentamicin (33.3%), imipenem (0%). All carried bla TEM , CTX-M-1 CTX-M-9 75% (18/24) SHV CTX-M-2 CTX-M-8 CTX-M-25 groups 20.8% (5/24) isolates. There co-occurrence CTX-M, β-lactamases 79.2% (19/24) isolates, five (20.8%) co-harbored CTX-M . showed high level multidrug gene carriage this study, suggesting likely review therapeutic options UTI clamp down on cases